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To knock down MST1 expression in INS1E cells, lentiviral shRNAmir targeting MST1 or control shRNAmir vectors (pGIPZ collection, Open Biosystems, Huntsville, Ala.) were transfected into INS-1E cells and stable clones were generated by selection with puromycine (1 to 2.5 μg/ml). Positive clonal cell lines were identified by immunoblotting using antibody directed against MST1. After selection

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Clarified cell lysates (20 µl) were used to determine luciferase activity using DualGlo reagents (40 µl, Promega) in a Veritas microplate luminometer (Turner Biosystems). All experiments were performed in duplicate and repeated on two different days.



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Open access articles that are associated with the species Homo sapiens and mention the gene name mir-200a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

Open biosystems pgipz manual arts
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17/12/2015 · For human knockdown studies, the pGIPZ system from Open Biosystems, Huntsville, Ala. was utilized. For knockdown of PAK1, the plko.1-puro vector system (Sigma) with non-silencing control or human PAK1 target was utilized. Lentiviral particles were produced utilizing 293T cells, and concentrated by ultracentrifugation. For overexpression studies, sorted LinKit+ cells from WT …

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Association between bisphenol A diglycidyl ether-specific

Clarified cell lysates (20 µl) were used to determine luciferase activity using DualGlo reagents (40 µl, Promega) in a Veritas microplate luminometer (Turner Biosystems). All experiments were performed in duplicate and repeated on two different days.

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Association between bisphenol A diglycidyl ether-specific

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-200a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

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Characterization of ATM Kinase-Dependent Signal

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This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is …

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Lentiviral shRNA vectors (pGIPZ, Open Biosystems) will be obtained for the interacting SDL partner genes. We will evaluate the SDL in 5 PTEN+ and 5 PTEN− breast tumor lines by transducing them with the lentiviral shRNA vectors and comparing the effects on growth after transduction with a control GFP reporter vector. In each case, the lentiviral-encoded shRNAs are appended to the GFP reporter

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